An efficient, fast, and integrated library preparation workflow that yields highly accurate data for sensitive sequencing applications.
Assay time
Hands-on time
Input quantity
Illumina DNA PCR-Free Prep offers a rapid and flexible workflow for preparing libraries for use in sensitive whole-genome sequencing applications, such as human whole-genome sequencing (WGS), de novo assembly of microbial genomes, and tumor–normal variant calling.
The Illumina DNA PCR-Free Prep workflow supports a broad DNA input range, multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots.
On-bead tagmentation chemistry combined with PCR-free library preparation eliminates PCR-induced bias and diminishes opportunities for error, providing superior and even coverage across high-GC or -AT regions. Learn more about on-bead tagmentation.
The Illumina DNA/RNA UD Indexes Sets offer up to 384 unique dual indexes, enabling accurate assignment of reads and efficient use of the flow cell.
Find fast, optimized sequencing library preparation solutions for use in a wide range of applications.
An efficient, fast, and integrated library preparation workflow that yields highly accurate data for sensitive sequencing applications.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Illumina DNA Prep with Enrichment
A complete targeted resequencing solution for a wide range of applications, with fast integrated workflow and options for custom and fixed enrichment panels.
| Assay time | ~1.5 hr |
|---|---|
| Automation capability | Liquid handling robot(s) |
| Automation details | Explore available automation methods |
| Description | Prepare sequencing libraries for sensitive applications using a unique combination of on-bead tagmentation with PCR-free chemistry which simultaneously eliminates PCR-induced bias and reduces total library prep time to 90 minutes. |
| Hands-on time | ~45 min |
| Input quantity | 25 ng to 300 ng |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System |
| Mechanism of action | Bead-linked transposome |
| Method | Whole-genome sequencing, De novo sequencing |
| Multiplexing | Up to 384-plex |
| Nucleic acid type | DNA |
| Specialized sample types | Blood, Not FFPE-compatible, Low-input samples, Saliva, Not FFPE-compatible |
| Species category | Any species, Human |
| Target insert size | 450 bp +/- 75 bp |
| Technology | Sequencing |
| Variant class | Short tandem repeats (STRs), Single nucleotide polymorphisms (SNPs), Somatic variants, Chromosomal abnormalities, Germline variants, Structural variants, Insertions-deletions (indels), Copy number variants (CNVs) |
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
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